Delivery Using Herpes Simplex Virus: An Overview

and Brown, J. This improvement was abolished when CD4(+) and CD8(+) lymphocytes were depleted, implying that the enhanced anti-tumor response to low-dose combined therapy is immune mediated. The effect of the deletion of Us9 was less severe in the NS Us9 deletion experiments. Together, our findings demonstrate that VP22 alone does not exert antitumor activity directly; however, this protein mediates the intercellular delivery of PTEN and thereby increases its intracellular concentration to achieve a therapeutic steady state, leading to an overall increase in the antitumor activity of PTEN. Pancreatic tumor cells injected intraperitoneally into nude mice resulted in significant tumor formation. However, a significant fraction of malignant tumours,8 including the high-grade glioblastomas,9 one of the main targets for therapy with HSVtk, poorly express connexin, a major gap junction protein, and thus exhibit little or no gap junction activity. Genome translocation is initially pressure-driven.

These results indicate that GAD gene therapy effectively suppresses DO after SCI predominantly through the activation of spinal GABA(A) receptors. The use of LPR resulted in a substantial increase of the gene expression level compared with the equivalent pDNA in the human lung cancer NCI-H460 carcinoma. Herpes simplex virus reactivation complicates labor in this group more often than in other obstetric patients. Western blot assessed sFlk-1 expression. In parallel, promoters have been developed that allow the long-term expression of individual or pairs of genes in DRGs by using elements from the latently active region of the virus to confer a long-term activity onto a number of promoters which otherwise function only in the short term. Although the survival rate of non-immunized mice treated with hrR3 alone was similar to that of mice treated with the hrR3/liposome complexes, the survival rates of immunized mice treated with hrR3 alone were significantly reduced compared to mice treated with the hrR3/liposome complexes. In addition, we have recently demonstrated that replication-defective HSV vectors expressing NT-3 are effective in improving erectile function in rats with chemically induced diabetes mellitus (DM) when the vectors were injected around the cavernous nerve 4 weeks after the induction of DM,39 although it is not known whether similar gene transfer techniques would be effective for the treatment of ED induced by nerve injury.


For example, if the current year is 2008 and a journal has a 5 year moving wall, articles from the year 2002 are available. Adorini, and G. We present evidence that VP22-EGFP fusion proteins were transported effectively from lentivirus transduced cells in vivo. Cell-penetrating peptides have recently attracted great interest in optimizing gene therapy, vaccine development and drug delivery. Delivery of an HSV vector expressing both green fluorescent protein (GFP) and lacZ (HSV-LacZ) was used as a control. Topical application without concurrent scarification also resulted in transgene delivery to 20% of the RPE cells of the rat but not the mouse. These persistent genomes are devoid of lytic gene expression, but retain the ability to express latency-associated transcripts (LATs).

Some dry skin or herpes assessment methods are worse than others: generally, people tend not to enjoy having a cotton swab inserted into any spray hole at all. 53 patients with recurrent HGG were randomly allocated to receive intra-arterial cerebral infusion of ADV-TK or conventional treatments. VP22 movement was observed both after delivery of DNA by transfection or microinjection and during virus infection. To determine the types of cells in the visual system of the mouse and rat that can express a transgene delivered by an attenuated replication competent Herpes simplex virus-1 (HSV-1) vector. HSV is highly infectious, so HSV vectors are efficient vehicles for the delivery of exogenous genetic material to cells. Purified recombinants or wild-type herpes simplex type 1 were injected into the left adrenal gland of hamsters. Deletion of both gamma(1)34.5 genes and inactivation of ICP6 (ribonucleotide reductase) allows G207 to selectively replicate within tumor cells.

Your library or institution may give you access to the complete full text for this document in ProQuest. Your library or institution may give you access to the complete full text for this document in ProQuest. BALB/c mice were given two intravenous injections of gD-ASOR, pBK-ASOR (plasmid lacking the gD-1 gene but complexed with ASOR), or PBS. Delivery of adenovirus, herpes simplex virus (HSV), and paramagnetic monocrystalline iron oxide nanoparticles (MION) to rat brain (n = 64) was assessed after intracerebral inoculation or osmotic disruption of the blood-brain barrier (BBB). Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. Mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) activity has been shown to suppress protein kinase R and thereby confer oncolytic susceptibility to some human tumors by R3616, a virus deleted for both copies of gamma(1)34.5. Epidural and subarachnoid opioids have been associated with the development of oral herpes simplex lesions.

(2001) Gene delivery and gene therapy with herpes simplex virus-based vectors.

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