Detection of cutaneous herpes simplex virus infections by immunofluorescence vs. PCR. – PubMed

The RIF test was type specific, easy to perform and gave diagnosis of HSV infections within an hour of taking the clinical specimen. Valaciclovir dose increasing from 1000 mg/day improved AES only moderately, but significantly promoted the incidence of DARs. Her infliximab was discontinued and she was started on acyclovir, 800 mg five times daily for 2 weeks. Cutaneous lumbosacral HSV remains uncommon in patients hospitalized with advanced diseases. Valaciclovir dose increasing from 1000 mg/day improved AES only moderately, but significantly promoted the incidence of DARs. We are not aware of any published reports of serious HSV infections associated with use of TNF inhibitors and cannot say whether treatment with infliximab, steroids alone, or the drug combination caused disseminated HSV-1 in our patient. In reactivated lesions epithelial syncytia and inclusion bodies were not seen; however, virus was demonstrable by polymerase chain reaction and culture.

9/24) (chi2 P = 0.000004) whereas electronmicroscopy was not (4/38 vs. Using PCR to specifically amplify HSV sequences which might be present, and then performing Southern analysis, we demonstrated HSV DNA in 9/13 HAEM and 6/9 IPEM biopsies. The limited activity of didemnins A and B coupled with irritation at the treatment site limits their usefulness in treating cutaneous herpesvirus infection. Fluid from skin vesicles examined by polymerase chain reaction showed Herpes Simplex Virus type 1. A hallmark of HSV infection is the ability of the virus to establish a persisting latent infection within sensory neurons. Vesicles were more likely to yield a positive culture than Tzanck smear (100% and 66.7%, respectively). Comparative studies between resveratrol cream, 10% docosanol cream (Abreva) and 5% acyclovir ointment (Zovirax) were also carried out.


We report the case of a young female with double immunosuppression (corticosteroids and azathioprine) due to Crohn’s disease, who developed a disseminated Herpes Simplex Virus (HSV) infection, resulting from a reactivation of latent HSV. DIF is a rapid, simple, sensitive, specific, cost-effective, and clinically useful technique for detecting and distinguishing cutaneous HSV and VZV infections. In summary, application of SP-303T ointment effected no significant improvement in the clinical course of 9 AIDS patients with acyclovir-unresponsive HSV infection. The difference between the positivity rates of the two tests was statistically significant. In vivo regulatory T-cells (Tregs) are implicated in the regulation of antiviral immunity and we have shown that signaling via the receptor activator of NF-κB (RANK) and its ligand RANKL mediates the peripheral expansion of Tregs. Skin diseases such as pemphigus, bullous pemphigoid, cutaneous T-cell lymphomas, and Sézary syndrome, Darier’s and Grover’s disease also predispose to severe cutaneous HSV or VZV infections. Approximately one-third of mice with primary skin lesions succumbed to neurologic disease and in the remaining mice cutaneous lesions healed completely.

The method of liposomal preparation rather than the lipid composition of the bilayers appeared to be the most important factor for reducing lesion scores. Two players experienced ocular lesions associated with cutaneous vesicular lesions of the face. This paper describes a nested multiplex polymerase chain reaction with respective sensitivities of 0.0001, 0.01 and 0.1 TCID50 for VZV, HSV-1 and HSV-2. The patient also had hypogammaglobulinaemia. The factors that trigger reactivation in humans are still poorly defined. Pregnant females and patients with impaired cellular immunity may be at increased risk, although healthy adults have been affected. Polymerase chain reaction (PCR) has been used to demonstrate the association, hence, is currently the only available sensitive diagnostic means for HSV-associated erythema multiforme.

The RIF test was evaluated for ease of use and speed of diagnosis. Polymerase chain reaction (PCR) has been used to demonstrate the association, hence, is currently the only available sensitive diagnostic means for HSV-associated erythema multiforme. The purpose of this report is to emphasize that cutaneous T-cell lymphoma is among the chronic skin disorders that may predispose to cutaneous dissemination of viral infections. Objective This study was conducted to compare the sensitivity for detection of HSV of an immunofluorescence method (Syva Microtrak) and an internally controlled PCR. A series of 111 thiosemicarbazones of 2-acetylpyridine, 2-acetylquinoline, 1-acetylisoquinoline, and related compounds were evaluated as inhibitors of herpes simplex virus in vitro and in a cutaneous herpes guinea pig model. Background  Cutaneous Herpes simplex virus (HSV) infections are regularly observed in lumbosacral areas, and many are refractory to appropriate initial diagnosis and management. Clinical reports suggest that stress precipitates recurrent cutaneous Herpes simplex virus (HSV) infection, presumably by reactivating latent infection in sensory ganglia with subsequent centrifugal axonal spread to the skin.

Please check the format of the address you have entered. Various studies have shown that major histocompatibility complex class I-restricted cytotoxic T lymphocytes (CTL) can be isolated from lymph nodes draining sites of cutaneous infection with herpes simplex virus type 1 (HSV-1). Cutaneous tests with herpes-antigens prepared from the infected chorio-allantoic fluid of chick-embryos were performed in children and adults. To investigate the role of epidermal Langerhans cells (LC) in resistance to herpes simplex virus (HSV) infection, nonadherent spleen cells taken from BALB/c mice immunized with HSV were cultured with syngeneic epidermal cells (EC) and ultraviolet light-inactivated HSV antigen.

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