Exposure Stress Induces Reversible Corneal Graft Opacity in Recipients With Herpes Simplex Virus-1 Infections

The atopic patients had a mean incidence of 0.07 episode/eye year (SD +/- 0.9) compared with 0.12/eye year (standard deviation [SD] +/- 0.21) in the nonatopics (P = .002). Forty-seven (76%) cases were culture positive for bacteria. However, protecting these grafts from exposure by tarsorrhaphy prevented or reversed the opacity in both syngeneic and allogenic grafts. Intraocular anti-HSV antibody production was detected in 9/28 AH samples tested (32%). However, protecting these grafts from exposure by tarsorrhaphy prevented or reversed the opacity in both syngeneic and allogenic grafts. However, in the absence of preexisting inflammation and vascularization, syngeneic grafts were accepted when the grafts were performed at a late time point after HSV infection (42 days), whereas allografts were rejected at this time. http://dx.doi.org/10.1590/S0004-27492002000500003.

However, in the absence of preexisting inflammation and vascularization, syngeneic grafts were accepted when the grafts were performed at a late time point after HSV infection (42 days), whereas allografts were rejected at this time. Postoperative best-spectacle-corrected visual acuity was 20/20 in 27 of 52 cases (52%) and at least 80% of patients achieved 20/30 at long-term follow-up. Objective refraction was +2.5/−1.25/55° OD and +1.5/−2.25/45° OS. Forty-seven (76%) cases were culture positive for bacteria. The death to culture time was 31 hours and the time in organ culture at 34°C was 18 days. The study confirms previous observations of HSV DNA in the corneal tissue of HSV seropositive patients apparently unrelated to any clinical manifestation of herpes infection. epidermidis, S.

The cornea is the clear window over the front of the eye and serves as the eye’s most powerful focusing lens. Informed consent was obtained from all the patients. Here we review the literature regarding corneal latency and the transmission of HSV-1. Median survival of the cohort was 27 months (95% confidence interval: 20.47-33.52). Initial indications for grafting in both groups were similar, other than keratoconus, which was a significantly more common indication in eyes that required only one graft. Influence of the amniotic membrane (AM) on the histology of severe HSV-1 necrotizing keratitis in BALB/c mice. It is anticipated that this new knowledge will allow development of specific molecular and genetic therapeutic strategies that could target critical steps in the immunopathogenesis of disease without the untoward side-effects of nonspecific generalized immune suppression that still remains the standard of care today.

Tissue sections were analyzed histologically for epithelialization and cellular infiltration and immunohistochemically with anti-CD3 mAb to T cells, anti-CD11b mAb to both macrophages and neutrophils, or anti-F4/80 mAb to macrophages. In certain conditions caused by either trauma or degenerative processes (Fuch’s Dystrophy), the cornea can become cloudy due to scarring or swelling. Slit-lamp examination of the left eye. Visual acuity, intraocular pressure, surgical complications, and recurrence were recorded. Most often, well-known reactivating stimuli could have caused the HSV infection. IFN-γ was detected in 19% (3/16), in the case of HSK only. This common disease is essentially a premature aging of the corneal endothelium.

The double-positive (CD31+/LYVE-1+) phenotype, indicating both HA and LA, was exhibited by 2 specimens (6%). Results In the control group we had only negative PCR results, in contrast to the corneas with severe or complete endothelial necrosis where HSV DNA was detected in 12 media of the corneas of 9 donors. Corneal status was inspected by the laser scanning confocal microscopy and anterior segment optical coherence tomography (AS-OCT). The donor cornea is held in place with an air bubble inside the eye. Eight patients experienced rejection episodes while on tacrolimus, and this led to graft failure in five patients. Most cataract operations performed have eyes that are otherwise normal apart from the cataract. Any problems within the cornea can lead to symptoms of blur due to either a lack of transparency or due to an irregular shape.

Therefore, the disease is spread by physical contact or by saliva. Herpes simplex virus type 1 keratitis was induced in BALB/c mice. 784 records were available for review of 1096 consecutive penetrating keratoplasty procedures performed between 1990 and 1999 (72%). The collagen fibers are in a multi-layered mesh that gives the cornea a defined shape and the ability to absorb some direct trauma. The selection of various procedures was based on the size and shape of corneal ulceration and perforation. Contact lenses, injuries, disorders, drugs, and nutritional deficiencies can cause open sores (ulcers) to form on the cornea. The middle of the cornea thins and gradually bulges outward producing increasing astigmatism, blurriness, possible swelling and scarring of the corneal tissue.

New developments in sutures, instruments, and microscopes, as well as changes in surgical techniques, have significantly improved the prognosis of patients requiring corneal transplantation. Inflammation, the first sign of wound healing, is also the hallmark of all ocular surface diseases. David Yorston Consultant Ophthalmologist, Gartnavel Hospital, 1053 Great Western Road, Glasgow G12 0YN,Scotland. * Final gross prices may vary according to local VAT.

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