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Serum samples from healthy controls and HIV-infected individuals were analyzed for IFABP using an Elisa kit according to manufactures instructions (Hycult Biotech Inc, Plymouth Meeting, Pennsylvania, USA). Nonlinear mixed-effects modeling was performed using a previously developed and extensively evaluated open 2-compartment population pharmacokinetic model of miltefosine, with first-order absorption and elimination from the central compartment as the structural base model [9, 10], using first-order conditional estimation with interaction between between-subject variability and residual error. Depending on their sexual risk behaviour in the preceding 6 months, attendees are further tested for the presence of anal CT and NG infections and pharyngeal NG infections. Because of this, causality cannot be determined. An STI-related episode was defined as an episode with an ICPC code for a positive STI-diagnosis or an episode with the ICPC code for fear of STI or HIV/AIDS (Table 1). The efficiency and effectiveness of screening also depends on the uptake and outcomes of therapy and other preventive measures that may follow from diagnosis. The staining procedure and scanning of the stained sections were performed according to the protocol described previously [40].

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